An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="8"><hr/></td></tr><tr class="thead"><td align="left">Compounds</td><td align="center">Q1</td><td align="center">Q3</td><td align="center">DP (V)</td><td align="center">EP (V)</td><td align="center">CEP (V)</td><td align="center">CE (V)</td><td align="center">CXP (V)</td></tr><tr><td class="thead-hr" colspan="8"><hr/></td></tr><tr><td align="left">Scopoletin</td><td align="center">193.2</td><td align="center">133.1</td><td align="center">42</td><td align="center">3</td><td align="center">20</td><td align="center">29</td><td align="center">3.9</td></tr><tr><td align="left">Umbelliferone</td><td align="center">163.1</td><td align="center">107.3</td><td align="center">38</td><td align="center">2</td><td align="center">11</td><td align="center">28</td><td align="center">2</td></tr><tr><td align="left">IS (digoxin)</td><td align="center">798.6</td><td align="center">113.3</td><td align="center">25</td><td align="center">3.8</td><td align="center">35</td><td align="center">68</td><td align="center">2</td></tr><tr><td align="left">Columbianetin</td><td align="center">246.8</td><td align="center">175.1</td><td align="center">61</td><td align="center">9</td><td align="center">16.8</td><td align="center">29</td><td align="center">3</td></tr><tr><td align="left">Psoralen</td><td align="center">187.2</td><td align="center">131.1</td><td align="center">42</td><td align="center">9</td><td align="center">20</td><td align="center">29</td><td align="center">10</td></tr><tr><td align="left">Bergapten</td><td align="center">217.4</td><td align="center">202.3</td><td align="center">44</td><td align="center">7</td><td align="center">15.8</td><td align="center">30</td><td align="center">3</td></tr><tr><td align="left">Xanthotoxin</td><td align="center">217.4</td><td align="center">174.1</td><td align="center">46</td><td align="center">8.8</td><td align="center">11</td><td align="center">34</td><td align="center">16</td></tr><tr><td align="left">Columbianetin acetate</td><td align="center">288.9</td><td align="center">229.1</td><td align="center">46</td><td align="center">2.9</td><td align="center">18</td><td align="center">12</td><td align="center">4</td></tr><tr><td align="left">Imperatorin</td><td align="center">271.3</td><td align="center">203.1</td><td align="center">49</td><td align="center">10</td><td align="center">31</td><td align="center">15</td><td align="center">17</td></tr><tr><td align="left">Osthole</td><td align="center">244.8</td><td align="center">189.2</td><td align="center">53.8</td><td align="center">3.7</td><td align="center">16.8</td><td align="center">20</td><td align="center">4</td></tr><tr><td align="left">Isoimperatorin</td><td align="center">271.3</td><td align="center">203.1</td><td align="center">29</td><td align="center">2</td><td align="center">17.6</td><td align="center">17</td><td align="center">3</td></tr><tr><td align="left">Columbianadin</td><td align="center">329.4</td><td align="center">229.2</td><td align="center">42</td><td align="center">3</td><td align="center">19.5</td><td align="center">32</td><td align="center">2</td></tr><tr class="table-tr"><td colspan="8"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Retention time, MS/MS fragment ions, declustering potential (DP), and collision energy (CE) of the 11 coumarins compounds in Radix Angelicae Pubescentis.

The Scientific World Journal

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Table 1

Table 1: An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations 

Table 1 | An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations 