An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations

Table 2

The calibration curves, LODs and LOQs () for the active components of the assay.

Compounds	Regression equation^(a)		Linear range	LOD^(b)	LOQ^(c)
			(ng mL^−1)	(ng mL^−1)	(ng mL^−1)
Scopoletin		0.9995	1–500	0.30	1.0
Umbelliferone		0.9992	10–1000	0.30	1.0
Columbianetin		0.9990	5–1000	0.50	1.7
Psoralen		0.9990	10–5000	0.33	1.0
Bergapten		0.9999	1.1–1100	0.30	1.0
Xanthotoxin		0.9990	10.7–1070	0.50	1.5
Columbianetin acetate		0.9992	13–1300	0.30	1.0
Imperatorin		0.9998	5–1000	0.02	0.2
Osthole		0.9998	12.7–1270	0.01	0.1
Isoimperatorin		0.9999	10.3–1030	0.01	0.1
Columbianadin		0.9990	13–1300	0.17	0.5

In the regression equation, the value is the concentration of compounds (ng·mL⁻¹), and the value is the peak area.
^(b)Limit of detection.
^(c)Limit of quantification.