An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations
Table 2
The calibration curves, LODs and LOQs () for the active components of the assay.
Compounds
Regression equation(a)
Linear range
LOD(b)
LOQ(c)
(ng mL−1)
(ng mL−1)
(ng mL−1)
Scopoletin
0.9995
1–500
0.30
1.0
Umbelliferone
0.9992
10–1000
0.30
1.0
Columbianetin
0.9990
5–1000
0.50
1.7
Psoralen
0.9990
10–5000
0.33
1.0
Bergapten
0.9999
1.1–1100
0.30
1.0
Xanthotoxin
0.9990
10.7–1070
0.50
1.5
Columbianetin acetate
0.9992
13–1300
0.30
1.0
Imperatorin
0.9998
5–1000
0.02
0.2
Osthole
0.9998
12.7–1270
0.01
0.1
Isoimperatorin
0.9999
10.3–1030
0.01
0.1
Columbianadin
0.9990
13–1300
0.17
0.5
In the regression equation, the value is the concentration of compounds (ng·mL−1), and the value is the peak area. (b)Limit of detection. (c)Limit of quantification.