The Scientific World Journal / 2015 / Article / Tab 3 / Research Article
An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations Table 3 Intra-assay and interassay precision of the developed method (
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Components Concentration Intra-assay Interassay Stability (ng mL−1 ) RSD (%) RSD (%) RSD (%) Scopoletin 20.6 5.03 6.17 9.46 103 3.16 5.20 5.44 1030 2.13 4.91 5.37 Umbelliferone 20 9.04 9.00 9.53 100 4.89 3.04 6.48 1000 5.14 7.11 9.35 Columbianetin 22 7.70 9.7 5.74 110 9.14 8.77 5.23 1100 2.93 8.62 8.47 Psoralen 21.6 3.61 9.11 8.05 108 5.28 9.07 6.40 1080 2.26 7.18 7.33 Bergapten 22 3.15 7.79 6.38 110 5.57 9.51 7.53 1100 3.38 8.35 7.45 Xanthotoxin 21.4 6.81 6.88 5.21 107 3.27 3.04 4.97 1070 2.87 8.31 7.88 Columbianetin acetate 26 8.05 3.24 9.04 130 7.84 7.59 6.05 1300 2.77 6.80 9.01 Imperatorin 20.6 4.32 2.42 5.62 103 8.48 3.78 6.08 1030 3.08 7.88 8.52 Osthole 25.4 5.48 6.77 8.08 127 3.72 5.49 2.75 1270 3.54 4.70 5.28 Isoimperatorin 20 4.17 3.81 5.19 100 2.98 7.05 6.68 1000 1.94 5.67 8.23 Columbianadin 26 9.28 8.56 9.73 130 2.90 1.45 5.84 1300 2.98 3.14 3.25