An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations

Table 3

Intra-assay and interassay precision of the developed method ().

Components	Concentration	Intra-assay	Interassay	Stability
	(ng mL^−1)	RSD (%)	RSD (%)	RSD (%)
Scopoletin	20.6	5.03	6.17	9.46
	103	3.16	5.20	5.44
	1030	2.13	4.91	5.37
Umbelliferone	20	9.04	9.00	9.53
	100	4.89	3.04	6.48
	1000	5.14	7.11	9.35
Columbianetin	22	7.70	9.7	5.74
	110	9.14	8.77	5.23
	1100	2.93	8.62	8.47
Psoralen	21.6	3.61	9.11	8.05
	108	5.28	9.07	6.40
	1080	2.26	7.18	7.33
Bergapten	22	3.15	7.79	6.38
	110	5.57	9.51	7.53
	1100	3.38	8.35	7.45
Xanthotoxin	21.4	6.81	6.88	5.21
	107	3.27	3.04	4.97
	1070	2.87	8.31	7.88
Columbianetin acetate	26	8.05	3.24	9.04
	130	7.84	7.59	6.05
	1300	2.77	6.80	9.01
Imperatorin	20.6	4.32	2.42	5.62
	103	8.48	3.78	6.08
	1030	3.08	7.88	8.52
Osthole	25.4	5.48	6.77	8.08
	127	3.72	5.49	2.75
	1270	3.54	4.70	5.28
Isoimperatorin	20	4.17	3.81	5.19
	100	2.98	7.05	6.68
	1000	1.94	5.67	8.23
Columbianadin	26	9.28	8.56	9.73
	130	2.90	1.45	5.84
	1300	2.98	3.14	3.25