An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations

Table 4

Repeatability and recovery of the developed method ().

Compounds	Reproducibility			Recovery^(a)
	Herbal	Capsule	Pill	Herbal		Capsule		Pill
	RSD (%)	RSD (%)	RSD (%)	Mean	RSD (%)	Mean	RSD (%)	Mean	RSD (%)
Scopoletin	4.97	6.32	6.72	96.1	5.74	105	1.85	101.	0.62
Umbelliferone	8.21	8.30	5.79	106	3.38	95.8	5.49	97.9	6.90
Columbianetin	4.07	9.53	5.31	110	4.09	99.3	2.69	102	0.59
Psoralen	4.71	7.86	3.81	99.8	5.66	97.2	6.44	105	8.46
Bergapten	4.09	6.41	4.56	106	5.67	108	0.47	101	6.95
Xanthotoxin	3.83	6.64	4.57	105	1.15	106	2.13	100	2.19
Columbianetin acetate	5.77	8.88	2.05	87.1	3.87	105	5.14	106	6.04
Imperatorin	3.72	9.12	3.69	107	1.87	104	2.60	105	3.82
Osthole	5.07	7.30	4.45	106	7.71	90.3	3.62	100	8.11
Isoimperatorin	5.10	6.48	7.16	106	1.64	107	0.32	105	3.28
Columbianadin	4.75	6.64	3.82	89.9	7.31	96.4	9.09	100	5.91

Recovery (%) = (Amount determined − Amount original)/Amount spiked × 100%.