The Scientific World Journal / 2015 / Article / Tab 4 / Research Article
An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations Table 4 Repeatability and recovery of the developed method (
).
Compounds Reproducibility Recovery(a) Herbal Capsule Pill Herbal Capsule Pill RSD (%) RSD (%) RSD (%) Mean RSD (%) Mean RSD (%) Mean RSD (%) Scopoletin 4.97 6.32 6.72 96.1 5.74 105 1.85
101. 0.62 Umbelliferone 8.21 8.30 5.79 106 3.38 95.8 5.49 97.9 6.90 Columbianetin 4.07 9.53 5.31 110 4.09 99.3 2.69 102 0.59 Psoralen 4.71 7.86 3.81 99.8 5.66 97.2 6.44 105 8.46 Bergapten 4.09 6.41 4.56 106 5.67 108 0.47 101 6.95 Xanthotoxin 3.83 6.64 4.57 105 1.15 106 2.13 100 2.19 Columbianetin acetate 5.77 8.88 2.05 87.1 3.87 105 5.14 106 6.04 Imperatorin 3.72 9.12 3.69 107 1.87 104 2.60 105 3.82 Osthole 5.07 7.30 4.45 106 7.71 90.3 3.62 100 8.11 Isoimperatorin 5.10 6.48 7.16 106 1.64 107 0.32 105 3.28 Columbianadin 4.75 6.64 3.82 89.9 7.31 96.4 9.09 100 5.91
Recovery (%) = (Amount determined − Amount original)/Amount spiked × 100%.