A Review of the Effects of Major Atmospheric Pollutants on Pollen Grains, Pollen Content, and Allergenicity

Figure 2

Two-dimensional gel electrophoresis analysis of the water-soluble proteins from Dactylis glomerata pollen harvested in ventilated greenhouse with air (a, c, e) or with air containing ozone, 100 μg/m³, 8 hours per day (b, d, f). Pollen extract from Dactylis glomerata was submitted to an initial isoelectrofocusing separation followed by gel electrophoresis with SDS. The gels were either silver stained (a, b) or transferred on nitrocellulose incubated with 2 different grass pollen-sensitized patient sera (c–f). IgE binding was revealed using heavy chain specific antibody coupled to alkaline phosphatase (AP). The AP activity was detected using 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium (Sigma) in 0.1 M tris buffer pH 9.5. Isoelectric points (pI) values (at the bottom) and relative molecular mass (kDa, on the right) are indicated for each gel.

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