The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana)

Table 3

Parameters of adsorption isotherms of bovine serum albumin on the adsorbent hydroxyapatite (HA), synthetic hydroxyapatite (HAS), and active babassu coal (ACB) at 25°C in pH 4.0 and pH 7.0 for the Langmuir and Freundlich models.

Model	Parameter	pH × adsorbents
		4.0			7.0
		HA	HAS	ACB	HA	HAS	ACB
Langmuir		87.95	68.26	36.18	57.82	55.90	30.90
		0.030	0.017	0.034	0.031	0.462	0.382
		0.9832	0.9886	0.9811	0.9915	0.9884	0.9937
	RMSE	1.34	0.34	1.43	1.03	1.55	0.12
Freundlich		81.55	65.84	25.04	42.16	36.34	28.81
		0.055	0.023	0.027	0.165	0.217	0.181
		0.9331	0.8449	0.9021	0.9043	0.9612	0.963
	RMSE	2.63	1.21	3.20	3.38	2.82	0.27

is maximum adsorption capacity (mg g⁻¹); dissociation constant is (mg g⁻¹) is depicting an affinity measurement. It is favorable if adsorption has value less than 1, indicating the tendency of the solute to migrate to the solid phase () dimensionless coefficient; the saturation capacity of the adsorbent is (mg g⁻¹); average error of the residue of the square (RMSE).