Chemical Profiling and Biological Activities on Nepalese Medicinal Plant Extracts and Isolation of Active Fraction of Nyctanthes arbor-tristis

Table 6

Isolation of active fractions from the extract of Nyctanthes arbor-tristis.

Eluting solvent system	Fraction no	Volume of eluent (mL)	TLC solvent system	TLC report
100% hexane	1–3	300	1% EtOAc in hexane	No spot
100% hexane	4–6	300	1% EtOAc in hexane	No spot
100% hexane	7–10	400	1% EtOAc in hexane	No spot
1% EtOAc in hexane	11–13	300	2% EtOAc in hexane	No spot
1% EtOAc in hexane	14–16	300	2% EtOAc in hexane	No spot
3% EtOAc in hexane	17–20	400	5% EtOAc in hexane	No spot
3% EtOAc in hexane	21–24	400	5% EtOAc in hexane	Tailing
5% EtOAc in hexane	24–28	500	10% EtOAc in hexane	One spot (tailing)
5% EtOAc in hexane	29–33	500	10% EtOAc in hexane	Tailing
5% EtOAc in hexane	34–38	500	10% EtOAc in hexane	Tailing
10% EtOAc in hexane	39–42	400	10% EtOAc in hexane	Tailing
10% EtOAc in hexane	43–46	400	10% EtOAc in hexane	Tailing
15% EtOAc in hexane	47–51	500	20% EtOAc in hexane	No clear spot
25% EtOAc in hexane	52–54	300	30% EtOAc in hexane	Two clear spots
25% EtOAc in hexane	55–58	400	30% EtOAc in hexane	No distinct spot
40% EtOAc in hexane	59–61	300	40% EtOAc in hexane	Tailing
40% EtOAc in hexane	62–64	300	40% EtOAc in hexane	Tailing
60% EtOAc in hexane	65–67	300	70% EtOAc in hexane	Tailing
60% EtOAc in Hexane	68–70	300	70% EtOAc in Hexane	Multi-spots
80% EtOAc in hexane	71–74	400	40% EtOAc in hexane	Tailing
100% EtOAc	75–81	600	1% MeOH in EtOAc	Tailing
1% MeOH in EtOAc	82–86	500	2% MeOH in EtOAc	Single spot
3% MeOH in EtOAc	87–91	500	5% MeOH in EtOAc	One spot (tailing)
5% MeOH in EtOAc	92–96	500	10% MeOH in EtOAc	Single spot (tailing)
10% MeOH in EtOAc	97–101	500	15% MeOH in EtOAc	One spot (tailing)
15% MeOH in EtOAc	102–105	400	25% MeOH in EtOAc	No clear spot
25% MeOH in EtOAc	106–110	500	30% MeOH in EtOAc	Tailing
50% MeOH in EtOAc	111–115	500	60% MeOH in EtOAc	Tailing
75% MeOH in EtOAc	116–120	500	80% MeOH in EtOAc	Tailing
100% MeOH	121–128	700	60% MeOH in EtOAc	Tailing