Detection of Mycobacterium avium subsp. paratuberculosis by a Direct In Situ PCR Method
Figure 1
(a) Positive in situ PCR signal (small blue spots) inside the cytoplasm of epithelioid and Langhans giant cells (lamina propria, ileum). (b) Positive in situ PCR signal (small blue spots) inside the cytoplasm of macrophages (ileocaecal lymph node, cortex). (c) Positive immunostaining (brown color) inside the cytoplasm of epithelioid cells. Tissue’s architecture is perfectly conserved (lamina propria, ileum). (d) Positive in situ PCR signal inside the cytoplasm of an epithelioid cell. Interpretation of this slide became very difficult due to damage on tissue’s architecture (lamina propria, ileum).
