Diagnosis and Molecular Characterization of Mycobacterium avium subsp. paratuberculosis from Dairy Cows in Colombia

Table 6

Comparison of diagnostic test results of single animals (𝑛=11) tested for Map in 2007 and in 2009

	2007^a					2009
Category	ELISA-A^b	ELISA-B^c	PCR^d	Real-time PCR^d	Culture	ELISA-C^e	Culture^f	PCR^g	Real-time PCR^g
1	+	−	−	+ (F)	−	+	−	−	−
2	+	−	+	+ (I)	−	−	−	N.D	N.D
3	+	+	−	−	−	−	−	N.D	N.D
4	+	−	+	−	−	−	−	N.D	N.D
5	+	−	−	−	−	−	−	N.D	N.D

^a According to Fernández-Silva et al. 2010 [10].
^b ELISA-A (Svanovir Para-TB Ab ELISA Kit, Svanova Biotech AB).
^c ELISA-B (ELISA paratuberculosis antibody verification, Institute Pourquier) performed only to positive and doubtful ELISA-A samples.
^d PCR and real-time PCR performed only to fecal samples from positive animals by ELISA-A.
^e ELISA-C (ID Screen Paratuberculosis Indiret, IDVET) performed to herds in the present study (2009).
^fCulture performed initially from pooled fecal samples, and then individually if pooled sample was Map positive.
^g PCR and real-time PCR performed only to individual fecal samples from culture-positive pooled samples, and to fecal samples from ELISA-C-positive animals regardless of culture result.
+: positive, −: negative, (F): positive real-time-PCR in marker F57, (I): positive real-time-PCR in marker ISMav2, ND: not done.