The First Report of Mycobacterium celatum Isolation from Domestic Pig (Sus scrofa domestica) and Roe Deer (Capreolus capreolus) and an Overview of Human Infections in Slovenia

Table 2

(a) An overview of laboratory diagnostics of mycobacteria in humans in Slovenia between 2000 and 2010

Year	No. of specimens	M. tuberculosis	M. bovis	M. caprae	M. bovis BCG	NTM^a	M. celatum
2000	17016	1022	0	0	0	180	0
2001	17576	1298	0	1	0	182	0
2002	16062	1178	0	0	2	170	2
2003	15236	1132	0	0	1	213	2
2004	12268	972	0	0	3	136	0
2005	11556	923	0	0	0	211	1
2006	11753	852	0	1	2	207	2
2007	11995	874	0	2	0	182	2
2008	11566	889	0	0	0	178	5
2009	11855	817	0	0	1	299	2
2010^b	12118	na^c	na^c	na^c	na^c	na^c	5
Total^b	149001	na^c	na^c	na^c	na^c	na^c	21

aNontuberculous mycobacteria.
bLaboratory diagnostics of specimens received in 2010 are still ongoing; as the final reports are not yet available at the time of writing, only the number of investigated specimens is given.
cNot available yet.

(b) Details on diagnostics of M. celatum from humans

Patient	Gender	Year of birth	No. of isolates	Year of isolation^1	Sample	Microscopy^2	Growth on media	Identification	Treatment
A	Female	1912	1	2002	Sputum	−^3	LJ^6	CM^10, BC^11, GT^12	No
B	Female	1947	1	2002	Sputum	−	MGIT^7	CM, BC, GT	No
C*	Female	1929	2	2003	Sputum	−	MGIT	CM, BC, GT	No
D	Male	1959	1	2005	Sputum	−	MGIT	CM, BC, GT	No
C*	Female	1929	2	2006	Sputum	−	MGIT	CM, GT	No
C*	Female	1929	2	2007	Sputum	±^4	LJ, ST^8, MGIT	CM, GT	No
C*	Female	1929	5	2008	Sputum	±^5	LJ, ST, MGIT	CM, GT	Yes
E	Female	1932	1	2009	Bronchial aspirate	−	Banič liquid medium^9	CM, GT	No
F	Male	1971	1	2009	Sputum	−	LJ, MGIT	CM, GT	No
C*	Female	1929	4	2010	Sputum	−	LJ, ST, MGIT	CM, GT	Yes
G	Female	1974	1	2010	Sputum	−	MGIT	CM, GT	No

*Patient with M. celatum pulmonary disease and a history of tuberculosis and M. avium and M. intracellulare-related pulmonary mycobacteriosis; confirmed as HIV-negative.
¹No M. celatum isolates were identified in years 2000, 2001, and 2004.
²Auramin fluorescent staining.
³Negative.
⁴One specimen positive and one specimen negative for acid-fast bacilli.
⁵Four specimens positive and one specimen negative for acid-fast bacilli.
⁶Löwenstein-Jensen medium.
⁷Mycobacteria Growth Indicator Tube (Becton Dickinson, USA), liquid medium.
⁸Stonebrink medium.
⁹ In-house liquid medium containing human serum (described in [22]).
¹⁰Colony morphology.
¹¹Biochemical tests (for details, see Table 3).
¹²GenoType Mycobacterium CM and AS culture identification kits (Hain Lifescience, Germany).