Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions

Table 1

Result summary from Experiment  1 after application of a pure Mycobacterium paratuberculosis culture to the top of 30 cm soil column of sandy loam soil from Indiana, USA.

Moisture content	Initial noculumsize (cfu ml^−1)	Week after bacterial inoculation
		1		2		3
		RT − PCR	Culture + IS900	RT − PCR	Culture + IS900	RT − PCR	Cultur + IS900
−0.1	10^8	+	−	−	−	+	−
−0.1	10^8	−	−	−	−	−	−
−0.1	10^7	−	−	−	−	−	−
−0.1	10^7	−	−	−	−	−	−
−0.03	10^8	−	−	−	−	+	−
−0.03	10^8	+	3+	−	−	+	−
−0.03	10^7	+	3+	−	−	−	−
−0.03	10^7	+	2+	+	−	+	−

Culture results were recorded as +, 2+, and 3+, which are equivalent to low, moderate, and high bacterial load on agar (<10 colonies-per-tube, (CPT), 10–50 CPT, and >50 CPT, resp.).