Intensity of Oestrus Signalling Is the Most Relevant Indicator for Animal Well-Being in High-Producing Dairy Cows

Table 1

Intra- and interassay measurements of relative P₄ concentrations [low (levels 1-2, <3.0 nM/L), medium (levels 3-4, 3.1–7.5 nM/L) or high (levels 5-6, >7.6 nM/L)), studied in fresh serum, plasma, or milk, or frozen-thawed serum or plasma samples, comparing different sample volumes, at various days (day 0, 7, or 20) of AI, provided by the eProCheck⁸⁰⁰. Number of observations per sample and coefficient of variation (CV) are indicated.

	Intraassay								Interassay
Sample status	Fresh				Frozen-thawed				Fresh	Frozen-thawed
Day sampled	d0	d20	d0	d7	d0		d20		d0	d0	d0
Sample type	Serum	Plasma	Milk	Milk	Serum	Plasma	Serum	Plasma	Serum	Serum	plasma
Sample size, μL	15	15	30	20	15	15	15	15	15	15	15
No. of replicates	5	10	10	7	7	7	7	7	7	6	7
Replicates	P4-Levels
1	low	med	low	high	med	high	high	high	low	low	high
2	low	med	med	high	high	high	high	high	low	low	high
3	low	med	low	high	med	high	high	high	low	low	high
4	low	high	med	high	high	high	high	high	med	low	high
5	low	med	low	high	med	high	high	high	low	low	high
6		med	med	high	med	high	high	high	low	low	high
7		med	med	high	high	med	high	high	low	low	high
8		med	low					high
9		med	med
10		med	low
CV (%)	<5.0	15.1	35.1	<5.0	22.0	13.2	<5.0	<5.0	33.1	<5.0	<5.0