The Role of Liver Biopsy in Detection of Hepatic Oxidative Stress

Table 1

Methods for preparation of liver biopsy implemented in different studies.

Tissue preparation	Buffer used	Homogenization	Oxidative stress marker	Reference
	Tris-HCL (50 mM) pH 7.5	The liver biopsy was homogenized in 20 volumes of cold buffer, and then the supernatant was harvested after centrifugation at 5000 g for 30 min at 4°C.	SOD, CAT and GSH	[24, 25]
	Chilled potassium chloride (1.17%)	Liver biopsy was homogenized in chilled buffer. The homogenates were centrifuged at 800 g for 5 min at 4°C to separate the nuclear debris. The obtained supernatant was recentrifuged at 10,500 g for 20 min at 4°C to get the postmitochondrial supernatant.	SOD, CAT and MDA	[68]
Liver biopsy samples were washed twice in cold 0.9% salt solution	Ice-cold PBS buffer (20 mM), pH 7.3 with 10 ml of 5 mM butylated hydroxyl toluene	The tissue was homogenized in 290 ml ice-cold buffer. Following this, the suspension was centrifuged and supernatant was fractioned for analysis.	LPO and AOP	[69]
	Tris-HCl (50 mM), pH 7.5, 5 mM EDTA, 1 nM dithiothreitol	The tissue was homogenized in 5 ml/g cold buffer. The homogenate was centrifuged at 10,000 g for 15 minutes at 4°C. The supernatant was removed for assay.	GSH-Px	[70]
	Potassium phosphate (0.05 M) and 0.1 mM EDTA, pH 7.8	The tissue was homogenized in 200 μL buffer and centrifuged at 15,000 g for 30 minutes at 4°C. The supernatant was used for analysis.	SOD	[70]