Table 2: Duration of M. bovis persistence in the environment on corn, hay, water and soil samples in season “A”, “B” and “C”.

Days to last positiveDays to 1st negative after last positive
Mean (standard deviation)Maximum (days)Mean (standard deviation)Maximum (days)

Fall/Winter “A”
 Corn24.00 (11.75)37.0029.75 (13.94)43.00
 Hay41.50 (3.00)*43.0054.25 (7.5)*58.00
 Soil21.75 (5.32)28.0028.75 (6.18)*37.00
 Water32.25 (18.23)58.0038.50 (14.80)58.00

All samples29.88 (12.88)58.0037.81 (14.59)58.00

Winter/Spring “B”
 Corn15.25 (7.89)*26.0024.50 (12.12)*42.00
 Hay26.00 (0.00)26.0042.00 (0.00)42.00
 Soil62.75 (30.63)*88.0068.25 (23.41)*88.00
 Water12.25 (10.50)*21.0018.25 (9.39)*26.00

All samples29.06 (25.56)88.0038.25 (23.61)88.00

Spring/Summer “C”
 Corn1.50 (1.73)3.007.00 (4.62)11.00
 Hay0.00 (0.00)0.003.00 (0.00)3.00
 Soil8.25 (5.50)11.0015.75 (8.50)20.00
 Water17.75 (22.01)*48.0023.50 (22.05)*53.00

All samples6.88 (12.48)48.0012.31 (13.53)53.00

*Significant difference in means (ANOVA) among substrate type Bonfferoni .