Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds

Table 2

Performances of isotypes/PPA-ELISAs for subclinically infected and clinically infected cattle.

Subclinically infected				Clinically infected
PPA	AUCs	Comparison of AUCs		PPA	AUCs	Comparison of AUCs
ELISA				ELISA
IgG	0.719	IgG ~ IgG1	𝑃=0.0858	IgG	0.998	IgG ~ IgG1	𝑃=0.0057
IgG1	0.526	IgG ~ IgG2	𝑃=0.2402	IgG1	0.805	IgG ~ IgG2	𝑃=0.0721
IgG2	0.812	IgG1 ~ IgG2	𝑃=0.0048	IgG2	0.927	IgG1 ~ IgG2	𝑃=0.0913

Estimated area under the curve (AUC) of each test and pairwise statistical analysis.