Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds

Table 3

Specificity and sensitivity of isotypes/PPA-ELISAs.

PPA	Specificity	Sensitivity
ELISA		Subclinically	Clinically	Total
IgG	100.0%	23.1%	92.9%	47.5%
IgG1	94.7%	11.5%	57.1%	27.5%
IgG2	92.1%	53.8%	85.7%	65.0%

The specificity of isotypes/PPA-ELISAs was calculated as % of cattle from the healthy control group testing negative. The sensitivity of each test was estimated as % of infected cattle (subclinically infected, clinically infected, or both) testing positive.