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Veterinary Medicine International
Volume 2012 (2012), Article ID 432763, 7 pages
http://dx.doi.org/10.1155/2012/432763
Research Article

Morphological Aspects and Immunophenotypic Profiles of Mammary Carcinomas in Benign-Mixed Tumors of Female Dogs

1Department of Medical Sciences, Federal University of Ouro Preto, Ouro Preto, MG, Brazil
2Faculty of Health Sciences, University of Vale do Rio Doce, Governador Valadares, MG, Brazil
3Laboratory of Investigative Pathology, Department of Anatomic Pathology, Hospital A.C. Camargo, São Paulo, SP, Brazil
4Laboratory of Comparative Pathology, Department of General Pathology, ICB-UFMG, 31270-901 Belo Horizonte, MG, Brazil

Received 8 March 2012; Accepted 8 August 2012

Academic Editor: Kazim Sahin

Copyright © 2012 Gustavo Meirelles Ribeiro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Carcinoma in benign-mixed tumor (CBMT) is common in the female canine mammary gland and comprises malignant epithelial between benign mesenchymal elements. This study investigated the morphological aspects of 29 CBMT and their immunophenotypical profiles, by using an immunohistochemistry panel based on five molecular markers—estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), cytokeratin 5 (CK5), and human epidermal growth factor receptor 1 (EGFR). From these, CBMT was classified into four subtypes: luminal A, luminal B, HER2-like, basal-like, and normal. “In situ” and invasive carcinomatous components were analyzed and compared. Histological grade I carcinoma was observed in 16 cases (55.2%) of the tumors analyzed, grade II in 10 cases (34.5%), and grade III in three cases (10.3%). The invasive carcinomatous component has shown, more frequently, luminal A (12/29 cases, 41.4%), followed by basal-like phenotype (8/29 cases, 27.6%). There was high concordance between immunophenotypical profiles of the in situ and invasive carcinomatous components (kappa coefficient = 0.816, 𝑃 < 0 . 0 0 1 ). We concluded that CBMT predominantly has features of low-grade neoplasms of malignancy. The various immunophenotypic profiles suggest the origin of these lesions in more than one cell type (luminal and myoepithelial).