T-Cell and Antibody Responses to Mycobacterial Antigens in Tuberculin Skin-Test-Positive Bos indicus and Bos taurus Cattle in Ethiopia

Table 1

List of mycobacterial antigens used the comparative study of immune response between B. indicus (Arsi breed) and B. taurus (Holstein) in Ethiopia.

Antigens	Protein	Peptides	Working concentration Final concentration at well (μg/mL)	Supplier
(1) ESAT-6-like:
CFP-10	X		5	Lionex^1
ESAT-6	X		5	Lionex
Rv0288	X		5	Protix^2
Rv0287	X		2	Dr Mustafa^3
Rv3019c	X		5	Lionex
(2) Heat-shock proteins:
HSP65	X		5	Lionex
HSP70	X		5	Lionex
Acr1	X		5	Lionex
Acr2	X		5	Protix
(3) Secreted antigens/lipoproteins:
Ag85A	X		5	Lionex
Ag85B	X		5	Lionex
MPB83	X		5	Lionex
MPB70	X		5	Lionex
19 kDa antigen	X		5	Lionex
38 kDa antigen (Pst-1)	X		5	Lionex
(4) Misc.
Rv0909		X	10	Pepscan^4
Rv3616c		X	10	Pepscan
Mb2555	X		5	VSD^5
Mb2890	X		5	Lionex
Mb3895		X	10	Pepscan
Rv3879c	X		5	VSD
Rv1196	X		2	Dr Mustafa
Rv1769	X		5	Lionex

¹Lionex, Braunschweig, Germany; ²Protix Prague, Czech Republic; ³Dr A. Mustafa, Kuwait University, Kuwait; ⁴Pepscan, Lelystad, The Netherlands; ⁵Veterinary Science Division (VSD), Belfast, Northern Ireland.