Opportunities for Improved Serodiagnosis of Human Tuberculosis, Bovine Tuberculosis, and Paratuberculosis

Table 1

Summary of humoral immune response based assays.

Target antigen	MTB/MB/MAP	Method of testing	Se (%)	Sp (%)	P	N	Species tested	Reference
PPD	MTB	ELISA	89	87	18	83	Human	[24, 25]
PPD	MB	ELISA	68–95	96–99	120	223	Cattle	[26, 27]
PPD	MAP	ELISA	29–72	99	359	2094	Cattle	[28, 29]
Antigen 5 (38 kDa)	MTB	ELISA	89	94–100	82	30	Human	[30, 31]
Cord factor	MTB	ELISA	81–84	96–100	65	66	Human	[32, 33]
ESAT-6	MTB	ELISA	67	51	100	100	Human	[34, 35]
ESAT-6	MB	ELISA	49–59	84–95	522	1489	Cattle	[36–38]
ESAT-6	MB	MAPIA	67	98	9	98	Deer
CFP-10	MTB	ELISA	48–63	51–71	100	100	Human	[34, 39, 40]
CFP-10	MB	ELISA	49–59	84–95	522	1489	Cattle	[36–38]
CFP-10	MB	MAPIA	56	99	9	98	Deer
Kp90	MTB	ELISA	78	82	51	71	Human	[41]
Antigen 60	MTB	ELISA	68–91	100	337	131	Human	[42, 43]
30 kDa antigen	MTB	ELISA	84	96.7	175	150	Human	[44, 45]
LAM	MTB	ELISA	17.8	87.7	47	153	Human	[27, 46–49]
LAM	MB	ELISA	60	na	120	—	Cattle
LAM	MAP	ELISA	66	88	167	216	Cattle
MS	MTB	ELISA	73–75	97-98	35	17	Human	[50–52]
MPT51	MTB	ELISA	80	na	53	—	Human	[51]
MPB70	MB	ELISA	73	88	120	223	Cattle	[27, 36–38, 53]
MPB70	MB	MAPIA	44	100	9	98	Deer
Antigen 85 complex	MB	ELISA	48	89	208	54	Cattle	[54, 55]
MPB 83	MB	ELISA	49–59	84–95	522	1489	Cattle	[36, 53, 56]
MPB83	MB	MAPIA	89	99	9	98	Deer	[36–38, 53, 56, 57]
MPB83	MB	RT	60	96	25	25	Cattle
MAP proteins 0862 and 3786	MAP	ELISA	81	na	11	—	Sheep	[58]
Ethanol extract	MAP	ELISA	97.4	100	64	38	Cattle	[59–62]
JTC	MAP	ELISA	56.3	99	444	412	Cattle	[63]

Se, Sensitivity; Sp, Specificity; P, no. of positive samples tested; N, no. of negative samples tested.