Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma

Table 1

Individual antibodies, vendor, titer, time of titration, epitope retrieval method, and method of detection.

Antibody	Manufacturer	Titer/Incubation (min)	Antigen retrieval Pretreatment	Detection
VDR	Santa Cruz Biotechnology, Inc, Santa Cruz, CA	1 : 500/30′′	None	Envision + anti-rabbit, Dako, Carpineria, CA
RXR Retinoid Receptor	Lab Vision Neomarkers, Fremont, CA	1 : 300/60′′	Citrate pH 6.0, Biocare Decloaking Chamber 5′′	Envision + anti-mouse, Dako, Carpinteria, CA
Ki-67 (MIB-1)	Dako, Carpinteria, CA	1 : 200/30′′	Citrate pH 6.0, Biocare Decloaking Chamber 5′′	Envision + anti-mouse, Dako, Carpinteria, CA
Survivin	Lab Vision, Fremont, CA	1 : 50/60′′	Citrate pH 6.0, Biocare Decloaking Chamber 5′′	Envision + anti-rabbit, Dako, Carpinteria, CA
Ezrin	Lab Vision, Fremont, CA	1 : 200/30′′	Citrate pH 6.0, Biocare Decloaking Chamber 5′′	Envision + anti-mouse, Dako, Carpinteria, CA