Table 1: Individual antibodies, vendor, titer, time of titration, epitope retrieval method, and method of detection.

AntibodyManufacturerTiter/Incubation (min)Antigen retrieval PretreatmentDetection

VDRSanta Cruz Biotechnology, Inc, Santa Cruz, CA1 : 500/30′′NoneEnvision + anti-rabbit, Dako, Carpineria, CA
RXR Retinoid Receptor Lab Vision Neomarkers, Fremont, CA1 : 300/60′′Citrate pH 6.0, Biocare Decloaking Chamber 5′′Envision + anti-mouse, Dako, Carpinteria, CA
Ki-67 (MIB-1)Dako, Carpinteria, CA1 : 200/30′′Citrate pH 6.0, Biocare Decloaking Chamber 5′′Envision + anti-mouse, Dako, Carpinteria, CA
SurvivinLab Vision, Fremont, CA1 : 50/60′′Citrate pH 6.0, Biocare Decloaking Chamber 5′′Envision + anti-rabbit, Dako, Carpinteria, CA
EzrinLab Vision, Fremont, CA1 : 200/30′′Citrate pH 6.0, Biocare Decloaking Chamber 5′′Envision + anti-mouse, Dako, Carpinteria, CA