Research Article

Search for Mycobacterium avium Subspecies paratuberculosis Antigens for the Diagnosis of Paratuberculosis

Table 1

Characterization in silico of a panel of 54 recombinant proteins, included in the present study.

Antigen (ORF no.)Predicted localizationTheoretical MWHomology with other mycobacteria

MAP 0011Cytoplasmic19,196 kDaIron-regulated peptidyl-prolyl cis-trans isomerase A in Mycobacterium tuberculosis H37Rv
MAP 0034Cytoplasmic-Membrane44 kDaP44 protein in Mycobacterium avium subsp. avium
MAP 0047cExtracellular41,1 kDaLpp-LpqN family conserved in Mycobacteriaceae
MAP 0038Unknown48,7 kDaHypothetical protein Mb0027 in Mycobacterium bovis AF2122/97
MAP 0187Extracellular23 kDaSuperoxide dismutase in Mycobacterium bovis
MAP 0209cExtracellular56,5 kDaProtein potentially involved in peptidoglycan biosynthesis in Mycobacterium avium subsp. avium
MAP 0210cCytoplasmic-Membrane 30,7 kDaSecreted antigen P36/P34 precursor in Mycobacterium bovis , maxima ident 60%
MAP 0211Cytoplasmic46 kDaUDP-galactopyranose mutase glf in Mycobacterium tuberculosis
MAP 0297Cytoplasmic55,23 kDaHypothetical protein Mb1161 Mycobacterium bovis AF2122/97
MAP 0334Unknown34,386 kDaOxidoreductase in Mycobacterium bovis AF2122/97
MAP 0900Cytoplasmic-Membrane29,6–34 kDaAntigen 34 kDa in Mycobacterium tuberculosis CDC1551
MAP 0946cCytoplasmic-Membrane33,5 kDaSigma factor in Mycobacterium avium 104
MAP 1012cCytoplasmic37,374 kDaHypothetical protein TMAG_01006 in Mycobacterium tuberculosis
MAP 1050cCytoplasmic33 kDaPeptidyl-prolyl cis-trans isomerase, cyclophilin-type conserved in Mycobacterium
MAP 1272Extracellular 33,4 kDaNLP/P60 family protein in Mycobacterium tuberculosis CDC1551
MAP 1293Unknown49,24 kDaHistidinol dehydrogenase his D in Mycobacterium tuberculosis
MAP 1308Unknown (This protein may have multiple localization sites.)46 kDaProlipoprotein diacylglyceryl transferase lgt in Mycobacterium tuberculosis
MAP 1564cUnknown23,01 kDaShort chain dehydrogenase in Mycobacterium bovis
MAP 1589cCytoplasmic21,60 KDaAlkyl hydroperoxide reductase subunit C in Mycobacterium tuberculosis
MAP 1653Unknown16,7–20 kDaThiol peroxidase tpx in Mycobacterium tuberculosis T17
MAP 1693cUnknown 18,30 kDaPeptidyl-prolyl cis-trans isomerase domain-containing protein conserved in Mycobacterium
MAP 1754cCytoplasmic-Membrane30,84 kDaHypothetical protein Rv2005c in Mycobacterium tuberculosis H37Rv
MAP 1889cCytoplasmic28 kDaWag31 protein in Mycobacterium avium 104
MAP 1962Cytoplasmic53,68 kDaGlutamine synthetase glnA1 in Mycobacterium tuberculosis
MAP 2020Unknown26,90 kDaCutinase in Mycobacterium bovis AF2122/97
MAP 2167Extracellular17 kDaLow molecular weight protein antigen cfp2 in Mycobacterium tuberculosis
MAP 2182Cytoplasmic-Membrane16 kDaDeazaflavin-dependent nitroreductase family protein in Mycobacterium
MAP 2513Cytoplasmic 36,50 kDaAlkanal monooxygenase alpha chain in Mycobacterium avium 104
MAP 2609Cytoplasmic-Membrane11,40 kDaLow molecular weight T-cell antigen TB8.4 in Mycobacterium tuberculosis
MAP 2676cCytoplasmic13,89 kDaHypothetical protein MAV_1246 in Mycobacterium avium 104
MAP 2685 Unknown21,20 kDaHypothetical protein BCG_1169c in Mycobacterium bovis BCG str. Pasteur 1173P2
MAP 2878cCytoplasmic25,43 kDaDihydrodipicolinate reductase in Mycobacterium bovis BCG str. Pasteur 1173P2
MAP 2942cExtracellular18,30 kDaSoluble secreted antigen MPT53 in Mycobacterium tuberculosis
MAP 2956Cytoplasmic30,02 kDa30S ribosomal protein S2 in Mycobacterium tuberculosis H37Rv
MAP 3175cCytoplasmic41,38 kDaPeptide chain release factor 2 in Mycobacterium tuberculosis H37Rv
MAP 3194Cytoplasmic30,46 kDaPyruvate carboxyl transferase in Mycobacterium avium
MAP 3205Cytoplasmic27 kDanuoE NADH dehydrogenase subunit E in Mycobacterium avium 104
MAP 3206Cytoplasmic48-49 kDanuoF NADH-Quinone oxidoreductase subunit F in Mycobacterium tuberculosis
Mb 3341cUnknown10,63 kDaEquivalent to Rv3312A, len: 103 aa, from Mycobacterium tuberculosis strain H37Rv
MAP 3402Cytoplasmic33,28 kDaThiosulfate sulfurtransferase in Mycobacterium bovis AF2122/97
MAP 3457Cytoplasmic47,61 kDa O-acetylhomoserine sulfhydrylase metC in Mycobacterium tuberculosis T92
MAP 3491Cytoplasmic28,16 kDaHydrolase in Mycobacterium tuberculosis SUMu003
MAP 3527Unknown35,70 kDaSerine protease PepA in Mycobacterium tuberculosis H37Rv
MAP 3627Cytoplasmic37,37 kDaO-methyltransferase in Mycobacterium tuberculosis H37Rv
MAP 3651Cytoplasmic44 kDaAcyl-CoA dehydrogenase fadE3 in Mycobacterium bovis BCG
MAP 3692Cytoplasmic47 kDafabG 3-ketoacyl-ACP reductase in Mycobacterium tuberculosis H37Rv
MAP 3840Cytoplasmic67 kDaHeat shock protein 70, molecular chaperone DnaK in Mycobacterium
MAP 3841Unknown23,57 kDaHeat shock protein GrpE in Mycobacterium bovis AF2122/97
MAP 3857Cytoplasmic18,73 kDaOrotate phosphoribosyltransferase phosphoribosyltransferase in Mycobacterium tuberculosis CDC1551
MAP 3936Cytoplasmic57 kDaHeat shock protein 65, GroEL in Mycobacterium sp
MAP 4000cUnknown12 kDaEsat-6 like protein esxF in Mycobacterium and hypothetical protein Mb3935c in Mycobacterium bovis
MAP 4143Cytoplasmic43,77 kDaIron-regulated elongation factor tu in Mycobacterium tuberculosis
MAP 4147Cytoplasmic42,09 kDaFerredoxin reductase in Mycobacterium tuberculosis H37Rv
MAP 4227cCytoplasmic30,14 kDaHypothetical protein Rv3463 in Mycobacterium tuberculosis H37Rv