Search for Mycobacterium avium Subspecies paratuberculosis Antigens for the Diagnosis of Paratuberculosis
Table 2
Spot intensities for protein macroarrays.
Antigen assayed
Mean spot intensity of proteins exposed to sera
control (n = 10)
PTB infected (n = 25)
TB infected (n = 8)
MAP 0011
112
135
0
MAP 0034
240
247
0
MAP 0038
141
860
0
MAP 0047
344
309
0
MAP 0187c
184
0
0
MAP 0209c
367
499
0
MAP 0210c
0
398
0
MAP 0211
0
572
0
MAP 0297
117
225
130
MAP 0334
108
167
1
MAP 0900
0
749
0
MAP 0946
218
900
295
MAP 1012
280
0
0
MAP 1050
268
275
0
MAP 1272
223
733
0
MAP 1293
585
481
396
MAP 1308
163
894
0
MAP 1564
259
344
0
MAP 1589c
323
682
466
MAP 1653
398
844
461
MAP 1693
226
967
209
MAP 1754
0
142
27
MAP 1889c
280
180
494
MAP1962
341
270
354
MAP 2020
0
150
228
MAP 2167
315
0
0
MAP 2182c
223
117
192
MAP 2513
41
164
291
MAP 2609
137
308
0
MAP 2676
201
138
0
MAP 2685
317
323
0
MAP 2878
307
323
0
MAP 2942
136
251
0
MAP 2956
345
119
424
MAP 3175
343
130
0
MAP 3194
0
100
0
MAP 3205
0
0
0
MAP 3206
100
200
0
Mb 3341
159
171
178
MAP 3402
205
178
250
MAP 3457
447
365
325
MAP3491
286
454
177
MAP 3527
528
414
635
MAP 3627
207
265
0
MAP 3651
1000
906
958
MAP 3692c
305
212
0
MAP 3840
0
198
712
MAP 3841
707
678
457
MAP 3857
373
267
265
MAP 3936
381
544
691
MAP 4000c
134
242
0
MAP4143
413
397
439
MAP 4147
1000
556
0
MAP 4227
138
101
0
PPDA
208
105
256
PPDB
346
531
1468
PPA-3
131
609
108
Cocktail
140
1460
0
Intensities were obtained using ImageQuant TL Array Version 7.0 Software, (GE Healthcare, Pittsburgh, PA). Antigens selected to evaluate as cocktail are shown in bold. PPA-3: Paratuberculosis protoplasmatic antigen (Allied Monitor, Inc. USA); PPDA and PPDB: avian- and bovine-derivative protein purified, respectively (Prionic Switzerland). Cocktail: mix of the 7 antigens shown in bold.
