316926.fig.001a
(a)
316926.fig.001b
(b)
Figure 1: Coding region of 10xM2e used for expression in L. lactis. (a) Protein and nucleic acid sequence of M2e from A/chicken/Pennsylvania/1370/1983/H5N2 is shown. (b) Nucleic acid sequence of coding region of 10xM2e used for expression in L. lactis is shown. The bases in italics were added to the ends of the coding region for 10xM2e. BspE1 sites (underlined) were used to clone the sequence in-frame into the unique BspE1 site in pip.