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Veterinary Medicine International
Volume 2013 (2013), Article ID 936317, 7 pages
http://dx.doi.org/10.1155/2013/936317
Research Article

Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog

1Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
2Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand

Received 5 June 2013; Accepted 28 August 2013

Academic Editor: Jyoji Yamate

Copyright © 2013 Korakot Nganvongpanit et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study examined the relationship between days of hip luxation and the expression of various mRNA. Twenty-six articular cartilages were used in the experiment: 3 samples were from normal dogs and 23 samples were collected from the femoral heads of hips that had been luxated for different lengths of time. Ten mRNA, including nonapoptotic genes (AGG, COL2A1, MMP-3, HAS-1, HAS-2, and TIMP-1) and apoptotic genes (BAX, BCL-2, CAS-3, and CAS-9), were studied for their expression using real-time PCR. We found very high correlation between expression level and luxation days ( ) in COL2A1, MMP-3, HAS-1, HAS-2, TIMP-1, BAX, and CAS-9, while the others (AGG, BCL-2, and CAS-3) also showed high correlation ( ). And we found a significant difference ( ) in the expression of transcripts depending on the number of luxation days. In conclusion, a delay in joint reduction may increase the chances of development of osteoarthritis.