982183.fig.002a
(a)
982183.fig.002b
(b)
Figure 2: (a) Agarose gel electrophoresis showing similar and divergent representative genetic profiles generated by LSSP-PCR from parasites that disseminate to different anatomical sites. (b) Phenetic analysis using the Jaccard coefficient of similarity and UPGMA algorithm. M: 100 bp DNA ladder marker; NC: negative control; PC: positive control (L. (V.) braziliensis reference strain MHOM/BR/1975/M2903). S-A8: spleen fragment from animal 8; PL-A8: popliteal lymph node fragment from animal 8; HS-A9: healthy skin fragment from animal 9; HS-A1: healthy skin fragment from animal 1; CL-A1: cervical lymph node fragment from animal 1; PL-A1: popliteal lymph node fragment from animal 1; LI-A8: liver fragment from animal 8; L-A8: lesion fragment from animal 8; L-A9: lesion fragment from animal 9; S-A9: spleen fragment from animal 9; L-A7: lesion fragment from animal 7; S-A7: spleen fragment from animal 7; L-A1 lesion fragment from animal 1.