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Veterinary Medicine International
Volume 2014 (2014), Article ID 150582, 5 pages
http://dx.doi.org/10.1155/2014/150582
Research Article

Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis

1The Postgraduate Program on Veterinary Medicine, FCAV-UNESP, Jaboticabal, SP, Brazil
2Departamento de Ciências Exatas, FCAV-UNESP, Jaboticabal, SP, Brazil
3Departamento de Clínica, Cirurgia e Reprodução Animal, FMVA-UNESP, Araçatuba, SP, Brazil
4Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV-UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n°, Bairro Industrial, 14.884-900 Jaboticabal, SP, Brazil

Received 26 August 2013; Revised 20 January 2014; Accepted 3 February 2014; Published 5 March 2014

Academic Editor: Guillermo Virkel

Copyright © 2014 Claudia Momo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to evaluate the most frequent lesions in the bone marrow of dogs naturally infected by Leishmania (Leishmania) chagasi. Thirty-three dogs sacrificed at the Zoonosis Control Center of Araçatuba, a municipality endemic for visceral leishmaniasis (VL), were used. The animals were classified as asymptomatic, oligosymptomatic, and symptomatic groups. At the necropsy, bone marrow samples were collected from the femur, fixed, processed, and stained with hematoxylin and eosin. The lesion intensity was classified as mild, moderate, or severe. The parasite load was determined using immunohistochemistry. The most important lesions consisted of multifocal to diffuse granulomas, megakaryocytic dysplasia, and medullary aplasia. There were no statistical differences between the three clinical groups regarding parasite load and lesion intensity. Asymptomatic dogs also presented high parasitism in the bone marrow as dogs with clinical signs of VL. It was concluded that, regardless of clinical group, the bone marrow is a site for multiplication of Leishmania chagasi. Possibly, the bone marrow dysplasia may arise from the presence of many parasitized and activated macrophages in this organ. Consequently, it affects the profile of hematopoietic cells in the bone marrow and systemic circulation.