Test M. farcinogenes M. senegalense References* Morphology and cultural characteristics Growth at 30°C + +
[56 ] Growth at 35–37°C + + [56 ] Growth after 3–5 days − + [11 , 15 , 56 ] Growth after 5–10 days + − [11 , 15 , 56 ] Colony wheat-colored + + [11 , 15 , 56 ] Colony convoluted + + [11 , 15 , 56 ] Colony rough + + [11 , 15 , 56 ] Colony very rough and grainy + − [11 , 56 ] Colony easily detached from agar + − [15 , 56 ] Colony relatively emulsifiable − + [11 , 56 ] Colony nonemulsifiable + − [56 ] Aerial hyphae sparse + + [15 , 56 ] Aerial hyphae abundant − − [15 , 56 ] Biochemical (enzyme) tests Acetamidase + + [11 , 15 , 82 ] Allantoinase +/− + [11 ] Arylsulphatase − + [15 , 76 , 82 ] Benzamidase +/− + [11 ] Catalase** + + [11 , 15 , 56 , 76 , 82 ] Iron citrate test − − [11 ] Isonicotinamidase +/− + [11 ] Beta-lactamase after 60 min + + [15 ] Neutral red test − − [11 ] Niacin production − − [11 ] Nicotinamidase +/− + [11 ] Nitrate reductase + + [11 , 15 , 76 ] Nitrophenol oxidase − − [15 ] Salicylamidase +/− + [11 ] Urease −/+ + [11 , 15 , 82 ] Urease − ND [76 ] Fluorogenic enzyme tests 4MU-
-L-arabinofuranoside + +/− [56 ] 4MU-
-L-arabinopyranoside + +/− [56 ] 4MU-
-D-fucoside + +/− [56 ] 4MU-
-D-galactoside + − [56 ] 4MU-
-D-glucoside − + [56 ] 4MU-
-D-glucoside + + [56 ] 4MU-p-guanidinobenzoate (HCl) + + [56 ] 4MU-
-D-mannopyranoside − + [56 ] 4MU-
-D-ribofuranoside + + [56 ] Degradation tests Aesculin + + [56 ] Arbutin + + [56 ] Casein − − [56 ] Elastin − − [56 ] Guanine − − [56 ] Hypoxanthine − − [56 ] Keratin − − [56 ] Testosterone + + [15 ] Tyrosine − − [15 , 56 ] Tweens + + [56 ] Xanthine − − [56 ] Growth in the presence of (%) Cobalt chloride (0.005) + + [56 ] Copper sulphate (0.01) + + [56 ] Crystal violet (0.001) − − [15 ] Ferrous sulphate (0.01) + + [56 ] Hydroxylamine HCl (0.05) − − [56 ] Lead acetate (0.01) + + [56 ] o-Nitrobenzoic acid (0.05) − + [56 ] Oleic acid (0.25, v/v) + + [56 ] Phenol (0.01) + + [56 ] Phenol (0.1) − − [15 , 56 , 82 ] Phenyl ethanol (0.02%) − + [15 , 82 ] Potassium tellurite (0.04%) − + [15 , 82 ] Potassium tellurite (0.5) − − [56 ] Pyronin G (0.1) − − [56 ] Sodium azide (0.005) − + [56 ] Sodium azide (0.01) − − [15 , 82 ] Sodium chloride (5) − − [56 ] Sodium chloride (5%) − + [15 , 82 ] Sodium deoxycholate (0.01) − + [56 ] Sodium nitrate (1) − − [56 ] Sodium salicylate (0.1) − + [56 ] Sodium selenite (0.001) − − [56 ] Thallous acetate (0.05) − − [56 ] Tetrazolium chloride (0.01) − − [56 ] Toluidine blue (0.03) − + [56 ] Teepol HB6 (0.05, v/v) − − [56 ] Zinc chloride (0.005) − − [56 ] Zinc chloride (0.01) − − [56 ] Growth at 45°C − − [15 , 76 ] pH4 − − [15 ] pH5 − + [15 , 82 ] pH10 − − [15 ] Survival at 60°C for 4 hours − − [15 ] Resistance to antibiotics and antibacterial agents (
gmL-1) Amikacin (2) − − [55 ] p-Aminosalicylic acid, Na salt (64) −/+ + [11 , 55 ] Amoxicillin (64) + + [55 ] Ampicillin (64) +/− + [55 ] Capreomycin sulphate (10) − + [15 ] Capreomycin sulphate (10) −/+ − [55 ] Cephaloridine (64) + + [55 ] Cephapirin Na salt (64) + + [55 ] Chlortetracycline HCl (64) −/+ −/+ [55 ] D-Cycloserine (2) + + [55 ] Cycloserine − − [11 ] Dapsone (16) + + [55 ] Dapsone (100) − + [15 , 82 ] Doxycycline HCl (8) − +/− [55 ] Doxycycline HCl (64) − − [55 ] Erythromycin (64) − + [55 ] Ethambutol HCl (4) + + [15 , 55 ] Ethambutol HCl (64) −/+ + [15 , 55 , 82 ] Ethionamide (5) + + [11 , 55 ] Gentamycin sulphate (128) − − [55 ] Isoniazid (2) + + [15 , 55 ] Kanamycin sulphate (2) + + [55 ] Kanamycin sulphate (16) − − [11 ] Lividomycin sulphate (16) − − [55 ] Lysozyme (50) + + [15 ] Lincomycin HCl (64) + + [55 ] Nalidixic acid Na salt (64) − + [55 ] Novobiocin (64) +/− + [55 ] Neomycin sulphate (128) − − [55 ] Oleandomycin phosphate (64) − + [55 ] Oxytetracycline HCl (64) − − [55 ] Paromomycin sulphate (64) − − [55 ] Penicillin (66 IU/mL) − +/− [15 ] Polymyxin B sulphate (64) − + [55 ] Prothionamide (10) + + [15 ] Rifampicin (16) + + [55 ] Rifampicin (20) + − [15 ] Streptomycin sulphate (1.6) + + [11 , 55 ] Streptomycin sulphate (64) − + [15 ] Sulphamethazine (1.6) + + [55 ] Thiacetazone (10) + −/+ [55 ] Trimethoprim + sulphamethoxazole (8) + + [55 ] Vancomycin HCl (64) − − [55 ] Viomycin sulphate (64) − − [11 , 55 ] Growth on sole carbon source (1%) Acetamide + + [56 ] Acetic acid (Na salt) + + [56 ] Butane 1,3 diol + − [15 , 82 ] Butane 2,3 diol − + [15 , 56 , 82 ] Ethanol − − [15 , 82 ] Fructose − + [56 ] D(+)Galactose − − [56 ] D-Gluconic acid + + [56 ] D(+)Glucosamine HCl − + [56 ] Hippuric acid (Na salt) − − [56 ] Lactic acid (Na salt) − + [56 ] Malonic acid (Na salt) − − [56 ] Maltose − − [56 ] Mannitol − + [56 ] Oxalic acid (Na salt) − − [56 ] 1,2-Propanediol − + [56 ] Pyruvic acid (Na salt) −/+ + [56 ] D(+)Raffinose − − [56 ] Rhamnose − + [56 ] Rhamnose − − [15 , 82 ] Salicin − + [56 ] Sucrose + − [56 ] Tartaric acid (Na salt) − − [56 ] Trehalose − + [56 ] D(+)Turanose − − [56 ]
