Table 2: Primers sequence for canine babesiosis according to Benson et al., (2002) [27].

PrimerSequenceReaction and/or useExpected amplicon

455-479FGTCTTGTAATTGGAATGATGGTGACSeminested PCR outer forward primer 340 bp
793-772RATGCCCCCAACCGTTCCTATTASeminested PCR outer reverse primer
BgibAsia-FACTCGGCTACTTGCCTTGTCSeminested PCR B. gibsoni (Asian genotype) specific forward primer185 bp
BCV-FGTTCGAGTTTGCCATTCGTTSeminested PCR B. c. vogeli specific forward primer192 bp
BCC-FTGCGTTGACGGTTTGACCSeminested PCR B. c. canis specific forward primer198 bp
BCR-FGCTTGGCGGTTTGTTGCSeminested PCR B. c. rossi specific forward primer197 bp