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Veterinary Medicine International
Volume 2014 (2014), Article ID 510328, 5 pages
http://dx.doi.org/10.1155/2014/510328
Research Article

Establishment, Culture, and Characterization of Guinea Pig Fetal Fibroblast Cell

1Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, 71348 Shiraz, Iran
2Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, 71348 Shiraz, Iran
3Department of Physiology, Islamic Azad University, Marvdasht Branch, 73985 Marvdasht, Iran

Received 24 December 2013; Revised 1 March 2014; Accepted 1 March 2014; Published 25 March 2014

Academic Editor: Jyoji Yamate

Copyright © 2014 Davood Mehrabani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Establishment of Guinea pig fetal fibroblast cells and their biological evaluation before and after cryopreservation were the main purposes of this study. After determination of the proper age of pregnancy by ultrasonography, 30 days old fetuses of Guinea pigs were recovered. Their skins were cut into small pieces (1 mm2) and were cultured. When reaching 80–90% confluence, the cells were passaged. Cells of the second and eighth passages were cultured in 24-well plates ( cells/well) for 6 days and three wells per day were counted. The average cell counts at each time point were then plotted against time and the population doubling time (PDT) was determined. Then, vials of cells ( cells/mL) were cryopreserved for 1 month and after thawing, the cell viability was evaluated. The PDT of the second passage was about 23 h and for the eighth passage was about 30 h. The viability of the cultures was 95% in the second passage and 74.5% in the eighth passage. It was shown that the Guinea pig fetal fibroblast cell culture can be established using the adherent culture method while, after freezing, the viability indices of these cells were favorable.