Evaluation of Sample Handling Effects on Serum Vitamin E and Cholesterol Concentrations in Alpacas

Table 1

Least squares means serum vitamin E concentrations (µg/dL) in 2 adult male alpacas after exposure of whole blood to the following conditions: exposure to fluorescent room light (light exposure: light or dark), contact with the rubber tube stopper (position: upright or stopper contact), storage duration before serum separation (time: 1, 4, or 24 hours), and temperature during storage (temp.: 4°C or 25°C). The effect of each alpaca was also analyzed. 𝑃 values are reported for the ANOVA 𝐹 statistic of the entire variable.

Variable	Mean (SE)	𝑃 value
Alpaca
A	44.3 (3.0)	0.25
B	48.5 (3.0)
Light exposure
Light	48.4 (4.0)	0.38
Dark	44.3 (2.3)
Position
Upright	57.5 (3.0)	<0.0001
Stopper contact	35.2 (3.0)
Time
1 hr	44.7 (3.5)	0.05
4 hr	52.8 (3.5)
24 hr	41.6 (3.5)
Temp.
4°C	46.4 (4.0)	0.99
25°C	46.3 (2.3)