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Veterinary Medicine International
Volume 2014 (2014), Article ID 850126, 7 pages
http://dx.doi.org/10.1155/2014/850126
Research Article

Staphylococcus pseudintermedius and Staphylococcus schleiferi Subspecies coagulans from Canine Pyoderma Cases in Grenada, West Indies, and Their Susceptibility to Beta-Lactam Drugs

1Pathobiology Academic Program, St. George’s University School of Veterinary Medicine, University Centre, Grenada
2Department of Veterinary Clinical Sciences, Ohio State University School of Veterinary Medicine, Columbus, OH 43210, USA
3Private Veterinary Practice, St. George, Grenada
4Small Animal Medicine and Surgery Academic Program, St. George’s University School of Veterinary Medicine, University Centre, Grenada

Received 8 October 2013; Revised 19 November 2013; Accepted 28 November 2013; Published 23 January 2014

Academic Editor: Pedro J. Ginel

Copyright © 2014 Harry Hariharan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Over a 2-year period 66 cases of canine pyoderma in Grenada, West Indies, were examined by aerobic culture in order to ascertain the bacteria involved and their antimicrobial resistance patterns. Of the 116 total bacterial isolates obtained, the majority belonged to Gram-positive species, and the most common organism identified through biochemical and molecular methods was Staphylococcus pseudintermedius. Additionally, identification of a Staphylococcus schleiferi subspecies coagulans isolate was confirmed by molecular methods. All isolates of staphylococci were susceptible to beta-lactam drugs: amoxicillin-clavulanic acid, cefovecin, cefoxitin, cefpodoxime, and cephalothin. They were also susceptible to chloramphenicol and enrofloxacin. Resistance was highest to tetracycline. Methicillin resistance was not detected in any isolate of S. pseudintermedius or in S. schleiferi. Among the Gram-negative bacteria, the most common species was Klebsiella pneumoniae, followed by Acinetobacter baumannii/calcoaceticus. The only drug to which all Gram-negative isolates were susceptible was enrofloxacin. This report is the first to confirm the presence of S. pseudintermedius and S. schleiferi subspecies coagulans, in dogs with pyoderma in Grenada, and the susceptibility of staphylococcal isolates to the majority of beta-lactam drugs used in veterinary practice.