Research Article

The Effects of an In Vitro Oocyte Maturation System and Chlorogenic Acid Supplementation during Embryo Culture on the Development of Porcine Cloned Embryos Derived from Native Vietnamese Ban Pigs

Table 1

Effects of maturation medium and hormone exposure duration on oocyte maturation and developmental competence of Ban Pig somatic cell nuclear transfer (SCNT) embryos.

IVM mediumsDuration of hormone supplementation (h)Number (%) of oocyteNumber (%) of SCNT embryos
CulturedWith a polar body (%)FusedCleaved (%)Developed to the blastocyst stage (%)

POM22329274 (83.6 ± 3.8)14987 (59.1 ± 5.1)30 (20.1 ± 3.3)
POM44307248 (81.4 ± 3.7)14383 (56.9 ± 4.3)18 (12.4 ± 2.4)
TCM22320242 (76.0 ± 3.8)14981 (53.4 ± 3.4)22 (14.3 ± 2.9)
TCM44318240 (76.0 ± 4.5)13579 (59.4 ± 3.9)22 (16.4 ± 2.8)

All experiments were repeated seven times. Percentage data are expressed as mean ± SEM.