The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality

Table 5

The effect of ETHEX on lipid peroxidation after 8, 24, 48 and 72 hours of ram liquid semen stored at 5°C in SM.

	Groups	Storage time (hours)
		8	24	48	72
SLPO	CONTROL	0.40 ± 0.02^bc	0.89 ± 0.04^a	1.75 ± 0.03^a	2.34 ± 0.03^ab
	ETHEX 1%	0.28 ± 0.02^c	0.38 ± 0.02^b	0.86 ± 0.04^d	1.35 ± 0.09^d
	ETHEX 2%	0.47 ± 0.05^b	0.80 ± 0.05^a	1.33 ± 0.09^c	1.63 ± 0.03^c
	ETHEX 4%	0.55 ± 0.07^ab	0.84 ± 0.05^a	1.54 ± 0.06^bc	2.13 ± 0.05^b
	ETHEX 8%	0.72 ± 0.03^a	0.88 ± 0.05^a	1.67 ± 0.04^ab	2.44 ± 0.07^a
ILPO	CONTROL	1.56 ± 0.12^a	1.66 ± 0.06^a	2.04 ± 0.03^a	3.40 ± 0.10^a
	ETHEX 1%	0.64 ± 0.03^c	0.75 ± 0.06^c	1.52 ± 0.08^b	1.78 ± 0.09^c
	ETHEX 2%	0.68 ± 0.05^c	1.33 ± 0.07^b	1.79 ± 0.06^ab	2.47 ± 0.08^b
	ETHEX 4%	1.08 ± 0.07^b	1.35 ± 0.06^b	2.03 ± 0.07^a	3.20 ± 0.06^a
	ETHEX 8%	1.05 ± 0.08^b	1.63 ± 0.04^a	2.06 ± 0.07^a	3.41 ± 0.08^a

SLPO: spontaneous LPO; ILPO: induced LPO. Values are expressed as mean ± SEM. Different superscripts^(a,b) within the same column indicate a significant effect of the extender within each duration of storage.