Florfenicol and Florfenicol Amine Quantification in Bull Serum and Seminal Plasma by a Single Validated UHPLC-MS/MS Method

Table 1

Selected mass transitions for florfenicol, florfenicol amine, and florfenicol-d3 and relative cone voltage and collision energy optimized values.

Analyte	Transitions monitored (m/z)	Cone voltage (V)	Collision energy (eV)
Florfenicol	355.9 ⟶ 335.9	40	8
	355.9 ⟶ 185.0	40	18
Florfenicol amine	248.0 ⟶ 130.0	40	21
	248.0 ⟶ 230.0	40	11
Florfenicol-d3	359.0 ⟶ 187.9	46	18
	359.0 ⟶ 338.9	46	8

Note: the product ion used for quantification is in bold.