Veterinary Medicine International / 2023 / Article / Tab 3 / Research Article
Florfenicol and Florfenicol Amine Quantification in Bull Serum and Seminal Plasma by a Single Validated UHPLC-MS/MS Method Table 3 Intra- and interday accuracy and precision data obtained for FF and FFA in bull serum and seminal plasma at four different QC concentrations in five replicates (n = 5), during three separated days of validation.
Serum Seminal plasma Accuracy (%) Precision (%) Accuracy (%) Precision (%) Florfenicol QC1 (0.05 μ g/mL) QC1 (0.05 μ g/mL) Day 1 (n = 5) 3.1 9.6 6.7 4.7 Day 2 (n = 5) 11.9 3.7 11.3 5.8 Day 3 (n = 5) −1.6 5.3 4.0 8.4 Interday (n = 15) 4.4 8.1 7.3 6.3 QC2 (0.25 μ g/mL) QC2 (0.25 μ g/mL) Day 1 (n = 5) 0.4 2.6 2.2 2.5 Day 2 (n = 5) 5.7 0.4 2.9 4.7 Day 3 (n = 5) 1.7 3.0 −0.9 3.6 Interday (n = 15) 2.6 3.1 1.4 3.7 QC3 (1 μ g/mL) QC3 (1 μ g/mL) Day 1 (n = 5) 3.8 0.9 0.9 1.4 Day 2 (n = 5) 2.6 1.2 0.4 1.4 Day 3 (n = 5) −0.8 4.2 −2.7 2.1 Interday (n = 15) 1.9 3.0 −0.5 2.3 QC4 (10 μ g/mL) QC4 (10 μ g/mL) Day 1 (n = 5) 2.2 2.4 3.0 3.2 Day 2 (n = 5) 2.9 1.3 3.5 2.0 Day 3 (n = 5) 4.6 0.5 2.5 1.9 Interday (n = 15) 3.3 1.7 3.0 2.1 Florfenicol amine QC1 (0.001 μ g/mL) QC1 (0.005 μ g/mL) Day 1 (n = 5) 10.0 12.9 13.3 7.3 Day 2 (n = 5) 2.3 8.8 8.0 11.3 Day 3 (n = 5) 8.3 8.6 6.7 7.6 Interday (n = 15) 6.9 8.8 9.3 8.2 QC2 (0.005 μ g/mL) QC2 (0.025 μ g/mL) Day 1 (n = 5) −8.7 1.9 −3.6 4.0 Day 2 (n = 5) 3.5 4.2 −0.3 5.1 Day 3 (n = 5) −3.3 3.0 7.3 3.1 Interday (n = 15) −2.9 6.0 1.2 6.0 QC3 (0.02 μ g/mL) QC3 (0.1 μ g/mL) Day 1 (n = 5) −6.0 2.7 −5.7 3.0 Day 2 (n = 5) 1.0 3.1 −3.1 3.2 Day 3 (n = 5) −5.3 3.2 −0.1 7.5 Interday (n = 15) −3.4 4.3 −3.0 5.1 QC4 (0.2 μ g/mL) QC4 (1 μ g/mL) Day 1 (n = 5) 5.5 0.2 3.5 3.8 Day 2 (n = 5) 2.5 1.4 5.0 2.2 Day 3 (n = 5) 0.6 3.3 −3.5 3.2 Interday (n = 15) 2.9 2.5 1.7 4.7