Florfenicol and Florfenicol Amine Quantification in Bull Serum and Seminal Plasma by a Single Validated UHPLC-MS/MS Method

Table 3

Intra- and interday accuracy and precision data obtained for FF and FFA in bull serum and seminal plasma at four different QC concentrations in five replicates (n = 5), during three separated days of validation.

	Serum			Seminal plasma
	Accuracy (%)		Precision (%)	Accuracy (%)		Precision (%)
Florfenicol
	QC1 (0.05 μg/mL)			QC1 (0.05 μg/mL)
Day 1 (n = 5)	3.1		9.6	6.7		4.7
Day 2 (n = 5)	11.9		3.7	11.3		5.8
Day 3 (n = 5)	−1.6		5.3	4.0		8.4
Interday (n = 15)	4.4		8.1	7.3		6.3
	QC2 (0.25 μg/mL)			QC2 (0.25 μg/mL)
Day 1 (n = 5)	0.4		2.6	2.2		2.5
Day 2 (n = 5)	5.7		0.4	2.9		4.7
Day 3 (n = 5)	1.7		3.0	−0.9		3.6
Interday (n = 15)	2.6		3.1	1.4		3.7
	QC3 (1 μg/mL)			QC3 (1 μg/mL)
Day 1 (n = 5)	3.8		0.9	0.9		1.4
Day 2 (n = 5)	2.6		1.2	0.4		1.4
Day 3 (n = 5)	−0.8		4.2	−2.7		2.1
Interday (n = 15)	1.9		3.0	−0.5		2.3
	QC4 (10 μg/mL)			QC4 (10 μg/mL)
Day 1 (n = 5)	2.2		2.4	3.0		3.2
Day 2 (n = 5)	2.9		1.3	3.5		2.0
Day 3 (n = 5)	4.6		0.5	2.5		1.9
Interday (n = 15)	3.3		1.7	3.0		2.1
Florfenicol amine
	QC1 (0.001 μg/mL)			QC1 (0.005 μg/mL)
Day 1 (n = 5)	10.0		12.9	13.3		7.3
Day 2 (n = 5)	2.3		8.8	8.0		11.3
Day 3 (n = 5)	8.3		8.6	6.7		7.6
Interday (n = 15)	6.9		8.8	9.3		8.2
	QC2 (0.005 μg/mL)			QC2 (0.025 μg/mL)
Day 1 (n = 5)	−8.7		1.9	−3.6		4.0
Day 2 (n = 5)	3.5		4.2	−0.3		5.1
Day 3 (n = 5)	−3.3		3.0	7.3		3.1
Interday (n = 15)	−2.9		6.0	1.2		6.0
	QC3 (0.02 μg/mL)			QC3 (0.1 μg/mL)
Day 1 (n = 5)	−6.0		2.7	−5.7		3.0
Day 2 (n = 5)	1.0		3.1	−3.1		3.2
Day 3 (n = 5)	−5.3		3.2	−0.1		7.5
Interday (n = 15)	−3.4		4.3	−3.0		5.1
	QC4 (0.2 μg/mL)			QC4 (1 μg/mL)
Day 1 (n = 5)	5.5		0.2	3.5		3.8
Day 2 (n = 5)	2.5		1.4	5.0		2.2
Day 3 (n = 5)	0.6		3.3	−3.5		3.2
Interday (n = 15)	2.9		2.5	1.7		4.7