Florfenicol and Florfenicol Amine Quantification in Bull Serum and Seminal Plasma by a Single Validated UHPLC-MS/MS Method

Table 4

Results of matrix effect (ME), recovery (RE), and process efficiency (PE) experiments in the two matrices, obtained from three replicates of each type of sample at the QC3 spike level of FF or FFA.

	Mean peak area (arbitrary units, ×10^3, n = 3)			ME (%)	RE (%)	PE (%)
	A	B	C
Florfenicol
Serum (1 μg/mL)	216.4	207.1	193.5	95.7	93.4	89.4
Seminal plasma (1 μg/mL)	186.9	172.3	164.6	92.2	95.5	88.0
Florfenicol amine
Serum (0.02 μg/mL)	11.4	10.8	9.6	94.7	88.6	84.2
Seminal plasma (0.1 μg/mL)	34.3	32.6	29.4	95.0	90.3	85.7

Note: (A = standard calibrators in mobile phase, containing the same amount of analytes as the third QC level; B = blank samples of each matrix fortified after extraction with the same amount of analytes as the third QC level; C = samples of each matrix fortified with the same amount of analytes as the third QC level and extracted)