International Journal of Proteomics

Review Article

Proteomics in Melanoma Biomarker Discovery: Great Potential, Many Obstacles

Table 1

Proteomics for biomarker discovery in melanoma.


Author	Year	Specimens	Comparison	Methodology	Proteins of interest

Bernard et al. [12]	2003	Cell lines	Melanocyte versus melanoma (primary and metastatic)	2DE and mass spectrometry	Nucleophosmin/B23, HDGF, CTSD
Sinha et al. [14]	2003	Cell lines	Responsive to chemotherapy versus nonresponsive	2DE and MALDI-TOF	Multiple (25)
Wilson et al. [28]	2004	Serum	Stage I or II melanoma patients, recurrence versus none	SELDI-TOF	n/a (expression profiles)
Carta et al. [13]	2005	Cell lines	Primary versus metastatic melanoma	2DE and mass spectrometry	HSP27, HSP60, HSPA8, PRDX2
Mian et al. [29, 30]	2005	Serum	Stage I melanoma versus Stage IV melanoma	SELDI-TOF	n/a (expression profiles), further work identified SAA
Mian et al. [29, 30]	2005	Serum	Stage III melanoma patients, recurrence versus none	SELDI-TOF	n/a (expression profiles), further work identified SAA
Takikawa et al. [24]	2009	Serum	Volunteers versus melanoma patients	Nano LC and MALDI-TOF	PPBP
Caron et al. [27]	2009	Serum	Volunteers versus melanoma patients	SELDI-TOF	n/a (expression profiles)
Greco et al. [31]	2009	Serum	Patients undergoing biopsy, melanoma versus not	2DE and MALDI-TOF	TTR, AGT, DBP
Paulitschke et al. [46]	2009	Secreted proteins from and cell lines and skin samples	Normal skin versus Melanoma	Nano LC and mass spectrometry	GPX5, periostin, stanniocalcini-1
Suzuki et al. [36]	2010	Serum	Volunteers versus melanoma patients	Autoantibody detection	EEF2, ENO1, ALDOA, GAPDH, HNRNP-A2B1
Hood et al. [47]	2010	Cell lines	Melanoma versus normal skin	Nano LC and mass spectrometry	Tenascin-C, fibronectin, ACN4, TSP-1
Liu et al. [37]	2010	Serum	Node negative versus node positive melanoma patients	Autoantibody detection	GRP94, ASAH1, CTSD, LDHB
Hardesty et al. [16]	2011	Surgically resected lymph nodes	Recurrence versus no recurrence	MALDI-IMS	Cytochrome C calmodulin

HDGF: hepatoma-derived growth factor, CTSD: cathespin D, PPBP: prop-platelet basic protein precursor, SAA: serum amyloid A, TTR: transthyretin, AGT: angiotensinogen, DBP: vitamin D binding protein, EEF2: eukaryotic elongation factor 2, ENO1: enolase 1, ALDOA: aldolase A, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, HNRNP-A2B1: heterogeneous nuclear ribonucleoprotein A2B1, ACN4: alpha-actinin-4, TSP-1: thrombospondin-1, GRP94: 94 kD glucose-regulated protein, ASAH1: acid ceramidase, LDHB: lactate dehydrogenase B.