Evolution of the reaction profile for I, cis-[Pt(¹⁵NH₃)₂I₂] with the DNA duplex R,S-5′-d(ATATGsGTATA)-3′·5′-d(TATACCATAT)-3′ containing a single phosphorothioate substitution, recorded as a function of time based on integration of 2D [¹H, ¹⁵N] HSQC NMR data acquired at a magnetic field strength of 14.1 T (¹H = 600 MHz). Key: blue diamond: cis-[Pt(¹⁵NH₃)₂I₂] starting material; red square: cis-[Pt(¹⁵NH₃)₂I(OH₂)]⁺ monoaqua, monoiodo adduct; green triangle: cis-[Pt(¹⁵NH₃)₂I,S]⁺ monoiodo, mono-phosphorothioate adduct; violet circle: cis-[Pt(¹⁵NH₃)₂S,G⁶-N7]²⁺ reaction end product.