The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis
Figure 6
Proposed intersubunit communication channel in bacterial ASADHs. The subunit interface of vcASADH (gray) is bridged by a hydrogen-bonding network (dotted lines) connecting the active sites of each subunit and stabilized by π-stacking interactions (dashed lines). In the subunit interface of mjASADH (green) replacement of Tyr160 with Met183 interrupts the hydrogen-bonding network, and replacement of Phe345 with Thr328 disrupts the π-stacking stabilization.
