Figure 4: Overexpression of CaCdr1p in a heterologous system. (a) Strategy showing the cloning and transformation of CaCDR1-GFP in S. cerevisiae. (b) Pictorial representation of the host AD1-8u showing the Deleted ABC pump proteins (pdr5, pdr10, pdr11, pdr15, snq2, yor1, ycf1) and the hyper expressed CaCDR1-GFP. (c) Topology of CaCdr1p. (d) Localization of CaCdr1-GFP in the host strain AD1-8u. The rimmed green fluorescent depicts overexpressing GFP tagged CaCdr1p.