Immunohistochemical detection of HCC biomarkers. (a) Liver tissue specimens from Sprague-Dawley rats treated with 1.25 mg/Kg of AFB. (b) Liver sample from patient with HCC without apparent exposure to AFB. (c) HCC case with detection of HBx protein, high expression, and nuclear localization. (d) Negative HCC case to HBx protein detection. (e) HCC case with detection of HCV Core protein, high expression in the cytoplasm of liver cells. (f) HCC case without expression of HCV core protein. Original magnification ×400.