The expression of CCL20 gene and protein by IL-17F in bronchial epithelial cells. (a) CCL20 gene expression by real-time PCR in BEAS-2B cells. Real-time PCR was performed as described in Materials and Methods. BEAS-2B cells were stimulated with IL-17F for 4 hrs (). (c) CCL20 protein levels in supernatants in BEAS-2B cells. ELISA was performed (). (d) CCL20 protein levels in supernatants in NHBEs (). * versus medium control. ** versus 10 ng/mL of IL-17F-stimulated cells. (e) CCL20 protein levels induced by IL-17A, IL-17E (IL-25), and IL-17F in supernatants in BEAS-2B cells (). * versus medium control. The values are expressed as .