Research Article
Mackerel Trypsin Purified from Defatted Viscera by Supercritical Carbon Dioxide
Table 1
Purification of trypsin (SC-1) from mackerel viscera defamed by SCO2.
| | Purification | Protein | Total | Specific | Purification | Yield | | stages | (mg) | activity | activity | (fold) | (%) | | | (U) | (U/mg) | | |
| | Crude enzyme | 1,390 | 1,049 | 0.8 | 1 | 100 | | Sephacryl S-200 | 502 | 946 | 2 | 3 | 90 | | Sephadex G-50 | 15 | 547 | 36 | 48 | 50 |
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