Research Article

Mackerel Trypsin Purified from Defatted Viscera by Supercritical Carbon Dioxide

Table 2

Effects of various inhibitors on the activity of SC-Ta.

InhibitorsConcentration% Inhibition

Control0
Soybean trypsin inhibitor1 mg/mL100
TLCK5 mM92
E-640.01 mM0
Pepstatin A0.01 mM0
EDTA2 mM0

a The enzyme solution was incubated with the same volume of inhibitor at 25°C for 15 min, and residual activity was analysed using TAME as a substrate for 5 min at pH 8.0 and 30°C.