Combined Use of a Solid-Phase Hexapeptide Ligand Library with Liquid Chromatography and Two-Dimensional Difference Gel Electrophoresis for Intact Plasma Proteomics
Figure 3
Effects of ProteoMiner treatment were examined by 2D-DIGE. The ProteoMiner-treated and untreated samples were labeled with Cy3 and Cy5, respectively, mixed, and separated by 2D gel electrophoresis. Note that a significant number of protein spots showed different intensities between the 2 samples. The dye-swapped images are shown in Supplementary Figure 2. (a) Original plasma; (b) flow-through fraction of HiTrap Blue HP column; (c) binding fraction of HiTrap Blue HP column. (d) Flow-through fraction of HiTrap Protein G HP column. (e) Binding fraction of HiTrap Protein G HP column; 0 mM fraction. (f) 100 mM fraction. (g) 150 mM. (h) 200 mM. (i) 250 mM. (j) 1 M fraction. (k) Resource Q column.