Review Article

Role of Charged Residues in the Catalytic Sites of Escherichia coli ATP Synthase

Figure 5

Loss of Pi protection from inactivation by NBD-Cl inhibition with inserted negative charge. Membranes were preincubated with Pi at zero, 2.5 or 10 mM concentration as shown, for 60 min at 23°C. Then, NBD-Cl (125 μM) was added and aliquots withdrawn for assay at time intervals as shown. ATPase activity remaining is plotted against time of incubation with NBD-Cl. ○, no Pi added; □, 2.5 mM Pi; Δ, 10 mM Pi. Data taken from [82, 86].
785741.fig.005a
(a)
785741.fig.005b
(b)
785741.fig.005c
(c)
785741.fig.005d
(d)
785741.fig.005e
(e)